DiaStar™ Direct Multiplex PCR Kit
For Direct PCR & Multiplex PCR without DNA extraction in samples
Multiplex PCR directly from whole blood without DNA extraction
Chemical-mediated Hot start enzyme
Amplicon size : ~ 1 kb
DiaStar™ Direct Multiplex PCR Kit is designed to perform PCR directly (or end-point multiplex) from whole blood, Plant, and mouse tail without prior DNA extraction. The product is based on 2X Smart mix that contains DNA polymerase, buffer and dNTP. The DNA polymerase in this mixture is a recombinant type of hot start PCR enzyme by improving A-family DNA polymerase. It has high specificity suitable for not only singleplex PCR but also multiplex PCR (maximum 20-plex). And the product minimize the reactions of PCR inhibitors in the blood so that enable to give high sensitivity and efficiency. (Enable to be amplified at maximum 1 kb with human whole blood)
Specification
| Source | A-family DNA p olymerase (Recombinant) |
Amplification fold | Yes (Chemical-mediated) |
| 3’A or Blunt ends | 3’A | ||
| 5’→ 3’exonuclease activity | Yes | Error rate | 12-13 bp error / 10⁶ bp |
| 3’→5’Exonuclease activity | No | Sample (template) type | Whole blood, Dried-blood (FTA card Sample) Plant seeds Mouse tail |
| Amplicon size | ~ 1 kb |
Application
Genetic testing, Multiplex PCR, PCR for genotyping, TA cloning
Format
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Smart mix | SBM01-K040 | DiaStar™ Direct Multiplex PCR Kit (for Blood) | 40 reaction | - |
| SBM01-K200 | DiaStar™ Direct Multiplex PCR Kit (for Blood) | 200 reaction | - | |
| DTM01-K040 | DiaStar™ Direct Multiplex PCR Kit (for Mouse tail) | 40 reaction | - | |
| DTM01-K200 | DiaStar™ Direct Multiplex PCR Kit (for Mouse tail) | 200 reaction | - | |
| DPM01-K040 | DiaStar™ Direct Multiplex PCR Kit (for Plant) | 40 reaction | - | |
| DPM01-K200 | DiaStar™ Direct Multiplex PCR Kit (for Plant) | 200 reaction | - |