[DR] DiaStar™ OneStep RT-PCR Series
For cDNA synthesis & PCR amplification at ‘One Step’ from RNA template
DiaStar™ OneStep RT-PCR Kit is a product for easy reverse transcription and PCR at “One Step” with optimal recombinant of DiaStarTM Reverse Transcriptase and Solg™ HotStart h-Taq DNA Polymerase mixture. This kit has dual advantages of high efficiency for cDNA synthesis and high specificity of hot start polymerase to set up of optimal PCR condition easily and conveniently
Specification
| Reverse transcription | Source | M-MLV (Recombinant) | RNase H activity | RNase H- |
| cDNA length | ~ 14 kb | |||
| Reaction temperature | 42 – 55 ℃ | Applicable Primers | Gene-specific primer | |
| PCR | Source | Thermus aquaticus (Solg™ h-Taq) | Hot start activity | Yes (Chemical-mediated) |
| Amplicon size | ~ 1 kb | 3’A or Blunt ends | 3’A |
Application
One-Step Multiplex RT-PCR, Gene expression analysis, RNA virus research, TA cloning
Citation&Papers
Chung EY, Kim BH,
et al., Resveratrol down-regulates interferon-γ-inducible inflammatory genes in macrophages: molecular mechanism via decreased STAT-1 activation, JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2010
Format
Ordering information
| Format | Cat. No. | Product | Size | Price (won) |
|---|---|---|---|---|
| Standard | DR61-K050 | DiaStar™ OneStep RT-PCR Kit | 50 reaction | - |
| DR61-K100 | DiaStar™ OneStep RT-PCR Kit | 100 reaction | - | |
| Smart mix | DR31-M10h | DiaStar™ 2X OneStep RT-PCR Smart mix, with dye² | 1 ㎖ (66 reaction)¹ |
- |
| DR31-M50h | DiaStar™ 2X OneStep RT-PCR Smart mix, with dye | 1 ㎖ x 5 ea | - | |
| Pre-Mix | DR31-P096 | DiaStar™ 2X OneStep RT-PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 96 tube (8 strip x 12 ea) |
- |
| Single item | DR65-B50h | DiaStar™ 5X OneStep RT-PCR Reaction Buffer | 1 ㎖ x 5 ea (833 reaction) |
- |