[SPD] Solg™ Pfu DNA Polymerase
For blunt-end PCR fragment amplification with high accuracy
High fidelity PCR
Amplicon size : ~ 3 kb
Solg™ Pfu DNA Polymerase is a recombinant type PCR enzyme, which has 12 times higher fidelity than other Taq DNA polymerase. It is recommended to use cloning for the purpose of protein expression which is required high fidelity. Produced target amplificon have blunt-end. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.
Specification
| Source | Pyrococcus furiosus (Recombinant) |
Amplicon size | ~ 3 kb |
| Hot start activity | No | ||
| 5’→ 3’ exonuclease activity | No | 3’A or Blunt ends | Blunt |
| 3’→ 5’ exonuclease activit y | Yes | Error rate | 1-2 bp error / 10⁶ bp |
Application
High fidelity PCR, Site-directed mutagenesis, Blunt-end cloning
Citation&Papers
Park AR and Oh DK,
Effects of galactose and glucose on the hydrolysis reaction of a thermostable β-galactosidase from Caldicellulosiruptor saccharolyticus, Applied Microbiology and Biotechnology , 85 : 1427-35, 2010
Kim ES, Hong BJ,
et al., Effects of lateral spacing on enzymatic on-chip DNA polymerization, Biosensors and Bioelectronics , 26 : 2566-73, 2011
Cho JI, Burla,
et al, Expression analysis and functional characterization of the monosaccharide transporters, OsTMTs, involving vacuolar sugar transport in rice (Oryza sativa), New Phytologist, 186 : 657-668, 2010
Format
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Standard | SPD16-R250 | Solg ™ Pfu DNA Polymerase | 250 U (200 reaction) |
- |
| SPD16-R500 | Solg ™ Pfu DNA Polymerase | 500 U | - | |
| SPD16-R25h | Solg ™ Pfu DNA Polymerase | 2,500 U (500 U x 5 ea) |
- | |
| SPD16-R50h | Solg ™ Pfu DNA Polymerase | 5,000 U (500 U x 10 ea) |
- | |
| Smart mix | SPD01-M50h | Solg ™ 2X Pfu PCR Smar t mix 1, with dye | 1 ㎖ x 5 ea (200 reaction) |
- |
| SPD02-M50h | Solg ™ 2X Pfu PCR Smart mix 2 (0.5X B and Doctor), with dye | 1 ㎖ x 5 ea | - | |
| Pre-Mix | SPD01-P096 | Solg ™ 2X Pfu PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 96 tube (8 strip x 12 ea) |
- |
| SPD01-P288 | Solg ™ 2X Pfu PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 288 tube (8 strip x 36 ea) |
- | |
| SPD01-P576 | Solg ™ 2X Pfu PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 576 tube (8 strip x 72 ea) |
- | |
| Single item | SPD22-B50h | Solg ™ 10X Pfu Reaction Buffer | 1 ㎖ x 5 ea (1,000 reaction) |
- |