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[SHT] Solg™ h-Taq DNA Polymerase

[SHT] Solg™ h-Taq DNA Polymerase

For High specific PCR amplification
Genotyping
Chemical-mediated Hot start enzyme
Ultra-high specificity
Amplicon size : ~ 1 kb

Solg™ h-Taq polymerase is chemically modified HotStart PCR enzyme, which enables multiplex and allele specific PCR for diagnostics and genotyping through initial heat activation during PCR cycling. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.

Specification

Specification에 관한 정보
Source Thermus aquaticus
(Recombinant)		 Amplicon size ~ 1 kb
Hot start activity Yes(Chemical-mediated)
5’→ 3’ exonuclease activity Yes 3’A or Blunt ends 3’A
3’→ 5’ exonuclease activit y No Error rate 12-13 bp error / 10⁶ bp

Application

Genotyping, Allele specific PCR, Highly specific amplification of genomic and cDNA targets, High G+C rich / Repeat region PCR, TA cloning

Citation&Papers

Lee GA, Kwon SJ,

et al., Cross-amplification of SSR markers developed from Allium sativum to other Allium species, Scientia Horticulturae , 128 : 401-407, 2011

Kee SK, Lee JY,

et al., Hypermethylation of the Ras Association Domain Family 1A (RASSF1A) Gene in Gallbladder Cancer, Molecules and Cells , 24 : 364-371, 2007

Lee HJ, Cha KE,

et al., In voitr Screening System for Hepatotoxicity: Comparison of Bone-Marrow-Derived Mesenchymal Stem Cells and Placenta-Derived Stem Cells,J ournal of Cellular Biochemistry, 112 : 49-58, 2011

Format

Ordering information

Format Cat. No. Product Size Price(won)
Standard SHT06-R250 Solg ™ h-Taq DNA Polymerase 250 U
(200 reaction)		 -
SHT06-R10h Solg ™ h-Taq DNA Polymerase 1,000 U
(250 U x 4 ea)		 -
SHT06-R25h Solg ™ h-Taq DNA Polymerase 2,500 U
(250 U x 10 ea)		 -
SHT06-R50h Solg ™ h-Taq DNA Polymerase 5,000 U
(250 U x 20 ea)		 -
Smart mix SHT01-M40h Solg ™ 2X h-Taq PCR Smar t mix 1, with dye 1 ㎖ x 4 ea
(160 reaction)		 -
SHT03-M40h Solg ™ 2X h-Taq PCR Smar t mix 2 (1.0X B and Doctor), with dye 1 ㎖x 4 ea -
Pre-Mix SHT01-P096 Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction 96 tube
(8 strip x 12 ea)		 -
SHT01-P288 Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction 288 tube
(8 strip x 36 ea)		 -
SHT01-P576 Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction 576 tube
(8 strip x 72 ea)		 -
Single item SHT21-B50h Solg ™ 10X h-Taq Reaction Buffer 1 ㎖ x 5 ea
(1,000 reaction)		 -
SHT95-E250 Solg™ h-Taq DNA Polymerase (only enzyme) 250 U
-

Manual

SHT01-M40h,_SHT06-M40h_Solg™_h-Taq_PCR_Smart_mix_1_개정일_20180706

SHT01-P096,_P288,_P576_Solg™_h-Taq_PCR_Pre-mix_개정일_20180706

SHT03-M40h,_SHT08-M40h_Solg™_h-Taq_PCR_Smart_mix_2_개정일_20180706

SHT06-R250,_R10h,_R25h,_R50h_Solg_h-Taq_DNA_Polymerase_20181119