[SUG] Solg™ E. coli Uracil-DNA Glycosylase
Removing DNA contamination in the air which could increase false-positive amplification
Solg™ E.coli Uracil-DNA Glycosylase(UDG) hydrolyzed the N-glycosylic bond between the Uracil base and sugar back bone. SolGent™ Uracil-DNA Glycosylase helps to get accurate diagnosis by removing of crossover or carry over DNA contamination which could increase false-positive amplification. In PCR using UDG system, it is used dUTP instead of dTTP. Please note that it can’t be used together with DNA Polymerase with proofreading function and dUTPase.
Specification
| Source | E. coli (K-12 strain) (Recombinant) |
Reaction temp. & time | 50 ℃ for 3 min |
| Inactivation | 95 ℃ for 5 min |
Application
Removing of uracil-containing DNA , Elimination carry-over contamination in PCR, PCR diagnosis, Site-directed mutagenesis
Citation&Papers
Choi JJ, Kim CH,
et al., Peptide Nucleic Acid-Based Array for Detecting and Genotyping Human Papillomaviruses, Journal of Clinical Microbiology, 47 : 1785-1790, 2009
Format
Ordering information
| Cat. No. | Product | Size | Price(won) |
|---|---|---|---|
| SUG21-R10h | SolGent™ E. coli Uracil-DNA Glycosylase | 1,000 U (1,000 reaction) |
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