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DiaPlexC™ MTB/M.Bovis Detection Kit

DiaPlexC™ MTB/M.Bovis Detection Kit

Multiplex PCR based assay system for simultaneous detection of MTB and M. bovis among MTC complex
CE-IVD

Mycobacterium tuberculosis (TB) is one of the most ubiquitously found dominant infectious agents around the world especially in developing countries that cause tuberculosis for almost 8 million and the death of 2 million people each year. Though there is a prevention method, the BCG (Bacillus Calmette-Guerin) TB vaccination, its disadvantage is that it cannot protect after 5 years from immunization and there is also possibility of diagnostic error for actual TB because there is a high chance of a false-positive result in the tuberculin test.

Specification

DiaPlexC™ MTB/M. Bovis Detection Kit
Detection target Mycobacterium tuberculosis (MTB), Mycobacterium bovis (M. bovis)
Registration CE-IVD
Detection technology Conventional (End-point) Multiplex PCR
Specimen type
Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum
Analytical sensitivity 10² copies
Compatible instruments* ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time ~ 2 hrs

Features

– HotStart PCR system : ultra high specific and sensitive result
– DnaFree™ system : No host genomic DNA contamination
– UDG system : No carryover contamination
– Multiplex PCR system : Multiple targets in a single reaction
– Reliable system : Automatic PCR control
– Positive control included
– Easy-to-use master mix
– CE certification

Experimental

Lane Interpretation (detection)
1 Negative (or NTC)
2 MTB
3 M. bovis
4 MTB, M. bovis (Co-infection)
5 Required re-experiment

Citation&Papers

“Mycobacterium tuberculosis” Sanger Institute.

2007-03-29 http://www.sanger.ac.uk/projects/M_tuberculosis/. Retrieved 2008-11-16

Frothingham R, Meeker-Meeker-O’Connell WA. (1998).

“Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats.”. Microbiology 144 (Pt 5): 1189-96.

Teo, SS ; Shingadia, DV (2006 Jun).


“Does BCG have a role in tuberculosis control and prevention in the United Kingdom?”. Archives of Disease in Childhood 91 (6) : 529-31.

Ordering information

Technology Cat. No. Product Contents
Conventional (End-point) PCR SMD22-K020 (20 reaction) DiaPlexC ™ MTB/M. Bovis Detection Kit 2X Multiplex PCR Smart mix (with UDG)
(MTB/M. Bovis)
Primer Mixture (MTB/M. Bovis)
Standard Marker (MTB/M. Bovis)
Control Template (MTB/M. Bovis)
Nuclease free Water
SMD22-K100 (100 reaction)