DiaPlexC™ MTB/M.Bovis Detection Kit
Multiplex PCR based assay system for simultaneous detection of MTB and M. bovis among MTC complex
CE-IVD
Specification
DiaPlexC™ MTB/M. Bovis Detection Kit |
|
---|---|
Detection target | Mycobacterium tuberculosis (MTB), Mycobacterium bovis (M. bovis) |
Registration | CE-IVD |
Detection technology | Conventional (End-point) Multiplex PCR |
Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum |
Analytical sensitivity | 10² copies |
Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended |
PCR running time | ~ 2 hrs |
Features
– HotStart PCR system : ultra high specific and sensitive result
– DnaFree™ system : No host genomic DNA contamination
– UDG system : No carryover contamination
– Multiplex PCR system : Multiple targets in a single reaction
– Reliable system : Automatic PCR control
– Positive control included
– Easy-to-use master mix
– CE certification
Experimental
Lane | Interpretation (detection) |
---|---|
1 | Negative (or NTC) |
2 | MTB |
3 | M. bovis |
4 | MTB, M. bovis (Co-infection) |
5 | Required re-experiment |
Citation&Papers
“Mycobacterium tuberculosis” Sanger Institute.
2007-03-29 http://www.sanger.ac.uk/projects/M_tuberculosis/. Retrieved 2008-11-16
Frothingham R, Meeker-Meeker-O’Connell WA. (1998).
“Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats.”. Microbiology 144 (Pt 5): 1189-96.
Teo, SS ; Shingadia, DV (2006 Jun).
“Does BCG have a role in tuberculosis control and prevention in the United Kingdom?”. Archives of Disease in Childhood 91 (6) : 529-31.
Ordering information
Technology | Cat. No. | Product | Contents |
---|---|---|---|
Conventional (End-point) PCR | SMD22-K020 (20 reaction) | DiaPlexC ™ MTB/M. Bovis Detection Kit | 2X Multiplex PCR Smart mix (with UDG) (MTB/M. Bovis) Primer Mixture (MTB/M. Bovis) Standard Marker (MTB/M. Bovis) Control Template (MTB/M. Bovis) Nuclease free Water |
SMD22-K100 (100 reaction) |