DiaPlexC™ Influenza Virus A Subtype Detection Kit
Multiplex PCR based assay system for identification of influenza virus A subtype
OneStep Multiplex RT-PCR
CE-IVD
Influenza virus A (H1N1) is a subtype of Influenza virus A and the most common cause of influenza (flu) in humans. Some strains of H1N1 are endemic in humans and cause a small fraction of all influenza-like illness and a large fraction Of all seasonal influenza. H3N2 is currently endemic in both human and pig populations. It evolved from H2N2 through an antigenic shift and caused the Hong Kong Flu pandemic of 1968 and 1969 that killed almost 750,000 people. The dominant strain of annual flu in January 2006 was H3N2. Since November 2003, nearly 400 cases of human infection with the highly pathogenic Avian Influenza A (H5N1) viruses have been reported by more than a dozen countries in Asia, Africa, the Pacific, Europe and the near East. Highly pathogenic Avian Influenza A (H5N1) virus infections occur in both poultry and humans. Furthermore, although H7N2, H7N3, H7N7 and H9N2 are classified as relatively low pathogenic infections, they have been reported in humans in the past few years and they can potentially develop into pandemic infections.
Specification
| DiaPlexC™ Influenza Virus A Subtype Detection kit |
||
|---|---|---|
| Detection target | Set I: H1N1-Seasonal, H1N1-Pandemic, H3N2 Set II: H5N1, H7 common, H9N2 |
|
| Registration | CE-IVD | |
| Detection technology | Conventional (End-point) OneStep Multiplex RT-PCR | |
| Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum |
|
| Analytical sensitivity | 10 - 10² copies | |
| Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended | |
| PCR running time | ~ 3 hrs | |
Features
– HotStart PCR system : Ultra high specific and sensitive result
– Reliable system : Automatic PCR control
– Positive control included
Experimental
| Lane | Interpretation (detection) |
|---|---|
| 1 | H1N1-Seasonal |
| 2 | H1N1-Pandemic |
| 3 | H3N2 |
| 4 | H5N1 |
| 5 | H7 common |
| 6 | H9N2 |
| 7 | Negative |
| 8 | NTC |
Citation&Papers
Grist NR, Bell EJ, Assaad F.
Enteroviruses in human disease. Prog Med Virol. 1978;24:114-57
Muir P, Nicholson F, Illavia SJ, et al.
Serological and molecular evidence of enterovirus infection in patients with endstage dilated cardiomyopathy. Heart. 1996;76(3):243-9.
Tebruegge M, Curtis N. Enterovirus infections in neonates.
Semin Fetal Neonatal Med. 2009;14(4):222-7.
Imamura T1, Fuji N, Suzuki A, et al.
Enterovirus 68 among children with severe acute respiratory infection, the Philippines. Emerg Infect Dis. 2011;17(8):1430-5.
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
| Real-Time PCR | SQD41-K020 (20 reaction) | DiaPlexQ™ Influenza Virus A Subtype Detection Kit | OneStep qRT-PCR Enzyme mix (Inf A Sub) 2X OneStep qRT-PCR Buffer (Inf A Sub) Primer & Probe Mixture I (Inf A Sub) Primer & Probe Mixture II (Inf A Sub) Control Template I (Inf A Sub) Control Template II (Inf A Sub) RNase free Water |
| SQD41-K100 (100 reaction) | |||
| Conventional (End-point) PCR | SMD41-K020 (20 reaction) | DiaPlexC™ Influenza Virus A Subtype Detection Kit | OneStep RT-PCR Enzyme mix (Inf A Sub) 2X OneStep RT-PCR Buffer (Inf A Sub) Primer Mixture (Inf A Sub) Standard Marker (Inf A Sub) Control Template (Inf A Sub) RNase free Water |
| SMD41-K100 (100 reaction) |