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DiaPlexQ™ Dengue Virus Detection Kit

DiaPlexQ™ Dengue Virus Detection Kit

Real-Time OneStep RT-PCR based assay system for detection of dengue virus
CE-IVD

Dengue Fever and Dengue hemorrhagic fever are caused by an RNA flavi virus that is spread by bites of mosquitoes, most commonly Aedes aegypti, which is found in tropic and sub-tropic regions. As many as 100 million people are infected yearly. There are four known serotypes of the flavi virus that cause Dengue Fever: DEN-1, DEN-2, DEN-3 and DEN-4. Infection from one serotype of Dengue Fever provides lifelong immunity from that serotype, but not from the other serotypes. There is no vaccines developed yet for Dengue Fever virus, it is essential to identify the strain of the infecting virus and the immunity status of the patient as early as possible.

Specification

DiaPlexQ™ Dengue Virus Detection Kit
Detection target Dengue Fever Virus (Serotype 1/2/3/4)
Registration CE-IVD
Detection technology Real-Time OneStep RT-PCR
Specimen type Blood
Analytical sensitivity 10 - 10² copies
Compatible instruments* ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
CFX96™ Real-Time PCR System (Bio-Rad)
PCR running time ~ 3 hrs

Features

– Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection
– HotStart PCR system : Ultra high specific and sensitive result
– Reliable system : Automatic PCR control
– Positive control included
– CE certification

Experimental

Sample type FAM Cy5 Result
Positive Control + + Valid
Negative Control - + Valid
NTC (Non-Template Control) - + Valid
Sample case 1 + +/- Positive
Sample case 2 - + Negative
Sample case 3 - - Required re-experiment

Citation&Papers

Grist NR, Bell EJ, Assaad F.

Enteroviruses in human disease. Prog Med Virol. 1978;24:114-57

Muir P, Nicholson F, Illavia SJ, et al.

et al. Serological and molecular evidence of enterovirus infection in patients with endstage dilated cardiomyopathy. Heart. 1996;76(3):243-9.

Tebruegge M, Curtis N. Enterovirus infections in neonates.

Semin Fetal Neonatal Med. 2009;14(4):222-7.

Imamura T1, Fuji N, Suzuki A, et al.

Enterovirus 68 among children with severe acute respiratory infection, the Philippines. Emerg Infect Dis. 2011;17(8):1430-5.

Ordering information

Technology Cat. No. Product Contents
Real-Time PCR SQD01-K020 (20 reaction) DiaPlexQ ™ Dengue Virus Detection Kit OneStep qRT-PCR Enzyme mix (Dengue)
5X OneStep qRT-PCR Buffer (Dengue)
Primer & Probe Mixture (Dengue)
Control Template (Dengue)
RNase free Water
SQD01-K100 (100 reaction)