DiaPlexC™ MTHFR Genotyping Kit
Multiplex allele-specific PCR based assay system for the genotyping of the MTHFR gene C677T and A1298C SNP related to hyperhomocysteinemia
CE-IVD
Specification
DiaPlexC ™ MTHFR Genotyping Kit | ||
---|---|---|
Detection target | Mutation C677T and A1298C of MTHFR gene | |
Registration | CE-IVD | |
Detection technology | Conventional (End-point) Multiplex PCR | |
Specimen type | Blood | |
Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended |
|
PCR running time | ~ 1 hr 30 min |
Features
– HotStart PCR system : Ultra high specific and sensitive result
– UDG system : No carryover contamination ( DiaPlexQ™)
– Multiplex PCR : Multiple targets in a single reaction
– Reliable system : Automatic Internal control ( DiaPlexC™)
– Positive control included
– Easy-to-use master mix
– CE certification
Experimental
Lane | Interpretation (detection) | Lane | Interpretation (detection) |
---|---|---|---|
1 | 677CC, 1298AA (Normal) | 6 | 677TT, 1298AC |
2 | 677CC, 1298CC | 7 | 677CT, 1298AA |
3 | 677CC, 1298AC | 8 | 677CT, 1298CC |
4 | 677TT, 1298AA | 9 | 677CT, 1298AC |
5 | 677TT, 1298CC | 10 | Required re-experiment |
Citation&Papers
Fodinger M, Horl WH, Sunder-Plassmann G (2000).
“Molecular biology of 5,10 methylenetetrahydrofolate reductase”. J Nephrol 13 (1): 20-33
Schneider JA, Rees DC, Lui YT, Clegg JB (May 1998).
“Worldwide distribution of a common methylenetetrahydrofolate reductase mutation”. Am. J. Hum. Genet, 62 (5): 1258-60
Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, Boers GJ, den Heijer M, Kluijtmans LA, van den Heuval LP et al. (May 1995).
“A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase”. Nat. Genet. 10 (1): 111-3.
Ordering information
Technology | Cat. No. | Product | Contents |
---|---|---|---|
Conventional (End-point) PCR | SHG31-K020 (20 reaction) | DiaPlexC™ MTHFR Genotyping Kit | 2X Multiplex PCR Smart mix (MTHFR) Primer Mixture (MTHFR) Standard Marker (MTHFR) Control Template (MTHFR) Nuclease free Water |
SHG31-K100 (100 reaction) |