DiaPlexQ™ 5 types Corneal Dystrophy (CD) Genotyping Kit
Real-Time PCR based assay system for the genotyping of the SNP of codon 124, 555(exon4) in TGFβ1 related to avellino corneal dystrophy
CE-IVD
Specification
Detection target | SNP of codon 124, 555 in TGFβI (R124H, R124L, R124C, R555Q, R555W) |
Registration | CE-IVD |
Detection technology | Real-Time PCR |
Specimen type | Blood, Buccal epithelial cell |
Compatible instruments* | ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) CFX96™ Real-Time PCR System (Bio-Rad) |
PCR running time | ~ 3hr |
Features
– HotStart PCR system : Ultra high specific and sensitive result
– UDG system : No carryover contamination
– Multiplex PCR : Multiple targets in a single reaction
– Reliable system : Automatic Internal control ( DiaPlex C™)
– Positive control included
– Easy-to-use master mix
– CE certification
Experimental
Color | Genotyping |
---|---|
Red | Wild-type |
Blue | Mutant type (ACD, RBCD, LCD, TBCD, GCD) |
Green | CD Hetero type |
Black | Non-Template Control |
Citation&Papers
Ridgway AEA, Moller HU (1992)
Genetics of granular dystrophy. Ophthalmology 99:175
Rogers C, Cohen P, Lawless M (1993)
Phototherapeutic keratectomy for Reis-Bucklers‘corneal dystrophy. Aust N Z J Ophthalmol 21:247–25
Munier FL, Korvatska E, Djema¨ı A, Le Paslier D, Zografos L, Pescia G, Schorderet DF(1997)
Kerato-epithelin mutations in four 5q31-linked corneal dystrophies. Nat Genet 15:247–25
Garner A.
Histochemistry of corneal granular dystrophy. Br J Ophthalmol 1969;53:799 805.
Akiya S, Brown SI.
Granular dystrophy of the cornea: characteristic electron microscopic lesion. Arch Ophthalmol 1970;84:179–92.
Owens SL, Sugar J. Edward DP.
Superficial granular corneal dystrophy with amyloid deposit. Arch Ophthalmol 1992;110:175–6.
Holland EJ, Daya SM, Stone EM, et al.
Avellino corneal dystrophy: clinical manifestations and natural history. Ophthalmology 1992;99:1564–8.
Ordering information
Technology | Cat. No. | Product | Contents |
---|---|---|---|
Real-Time PCR | SQH29-K020 (20 reaction) | DiaPlexQ ™ 5 type Corneal Dystrophy(CD) Genotyping Kit | 2X Multiplex Real-Time PCR Smart mix (CD) Primer & Probe Mixture (CD) Wild-type Control Template (CD) Hetero-type Control Template (CD) Mutant-type Control Template (CD) Nuclease free Water |
SQH29-K100 (100 reaction) |