DiaPlexQ™Apolipoprotein E (ApoE) Genotyping Kit
Real-Time PCR based assay system for the genotyping of the ApoE gene SNP related to alzheimer’s disease
CE-IVD
Specification
DiaPlexQ™ ApoE Genotyping Kit | ||
---|---|---|
Detection target | ApoE genotype (Mutation T112C and T158C of ApoE gene) E2/E2, E3/E3, E4/E4, E2/E3, E2/E4, E3/E4 |
|
Registration | CE-IVD | |
Detection technology | Real-Time PCR | |
Specimen type | Blood |
|
Compatible instruments* | ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) CFX96™ Real-Time PCR System (Bio-Rad) |
|
PCR running time | ~ 1 hr 30 min |
Features
– HotStart PCR system : Ultra high specific and sensitive result
– UDG system : No carryover contamination ( DiaPlexQ™)
– Multiplex PCR : Multiple targets in a single reaction
– Reliable system : Automatic Internal control ( DiaPlexC™)
– Positive control included
– Easy-to-use master mix
– CE certification
Experimental
ApoE genotype | SNP | 112 site | 158 site |
---|---|---|---|
E2/E2 | TT/TT | T/T type | T/T type |
E2/E3 | TT/TC | T/T type | T/C type |
E2/E4 | TC/TC | T/C type | T/C type |
E3/E3 | TT/CC | T/T type | C/C type E4 |
E3/E4 | TC/CC | T/C type | C/C type E4 |
E4/E4 | CC/CC | C/C type E4 | C/C type E4 |
Non-Template Control |
Citation&Papers
Lepara O, Valjevac A, Alajbegovic A, Zaciragic A, Nakas-Icindic E (August 2009).
Decreased serum lipids in patients with probable Alzheimer’s disease”. Bosn J Basic Med Sci 9 (3): 215-20.
Golomb BA, Evans MA (2008).
“Statin adverse effects : a review of the literature and evidence for a mitochondrial mechanism”. Am J Cardiovasc Drugs 8 (6): 373-418
Ordering information
Technology | Cat. No. | Product | Contents |
---|---|---|---|
Real-Time PCR | SQH01-K020 (20 reaction) | DiaPlexQ ™ ApoE Genotyping Kit | 2X Multiplex Real-Time PCR Smart mix (ApoE) Primer & Probe Mixture (ApoE 112) Primer & Probe Mixture (ApoE 158) T/T type Control DNA (ApoE 112) T/C type Control DNA (ApoE 112) C/C type Control DNA (ApoE 112) T/T type Control DNA (ApoE 158) T/C type Control DNA (ApoE 158) C/C type Control DNA (ApoE 158) Nuclease free Water |
SQH01-K100 (100 reaction) | |||