[SDL] SolGent™ T4 DNA Ligase
Ligation enzyme Connection of cohesive end / blunt end PCR product
SolgTM T4 DNA Ligase is a ligation enzyme and it is designed to connect and combine 5’-phosphate and 3’-OH phosphodiester by using of ATP as a cofactor which is laid in a parallel. This product is useful to connect both of cohesive end and blunt end PCR product. SolgTM T4 DNA Ligase is used to connect nick which is formed in a single strand.
Specification
| Source | Bacteriophage T4 (Recombinant) |
Ligation temp. & time | 16 ℃ for 2 hr or 20 ~ 25 ℃ for 1 hr |
| Inactivation | 70 ℃ for 10 min |
Application
Cohesive or Blunt-end ligation, Cloning of r estriction fragments, Joining linkers and adapters to blunt-ended DNA, Repairing nicks (in duplex DNA, RNA or DNA-RNA h ybrids)
Format
Ordering information
| Cat. No. | Product | Size | Price(won) |
|---|---|---|---|
| SDL01-R40k | SolGent™ T4 DNA Ligase | 40,000 U (100 reaction) |
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