[SCA] SEQ-TempliGen RCA™ Kit
Low-copy plasmid, BAC, Fosmid Amplification Kit
SEQ-TempliGen™ RCA Kit is a product for plasmid DNA amplification from small amount of various sample including liquid culture, colony, glycerol stock, and purified DNA based on Rolling Circle Amplification (RCA) mechanism. RCA mechanism is one of the isothermal amplification methods, which is used circular DNA as template by activity of Phi29 DNA polymerase at 30 ℃. Amplified product can be used for sequencing directly without additional purification step.
Specification
| Source | Bacillus subtilis phage Phi29 | Amplification fold | 10³ ~ 10⁹ |
| 5’→ 3’exonuclease activity | No | Error rate | 1 bp error / 10⁶ - 10⁷ bp |
| 3’→5’Exonuclease activity | Yes | Sample (Template) | Colony*, liquid culture, glycerol Strand displacement activity Yes stock, purified DNA |
| Strand displacement activity | Yes | ||
| Reaction temperature | 30 ℃ | Amplification Time | ~2 hours |
Application
Colony direct amplification for sequencing, Low-copy plasmid amplification, Library amplification, High fidelity replication at moderate temperatures
Citation&Papers
Hien TT, Park HD,
et al., Influence of Different Substrates in Wetland Soils on Denitrification, WATER AIR AND SOIL POLLUTION, 215 : 549-560, 2011
Format
Ordering information
| Cat. No. | Product | Size | Price(won) |
|---|---|---|---|
| SCA60-K200 | SEQ-TempliGen™ RCA Kit | 200 reaction | - |