[DR] DiaStar™ RT Series
For cDNA synthesis with high sensitivity & high efficiency from Template RNA
Diastar™ RT Kit is a thermostable mutant of M-MLV Reverse Transcriptase (Rnase H-) which is recombinant type. This mutant type of Rnase H- enables more efficient cDNA synthesis due to more thermostable characteristic (optimal activity at 50 ℃).
Specification
| Source | M-MLV (Recombinant) | H Gene-specific primer - | ~ 14 kb |
| Reaction temperature | 42 – 55 ℃ | Applicable Primers | Random primer, Oligo d(T) primer, Gene-specific primer |
| RNase H activity | RNase H- |
Application
First-strand cDNA synthesis, Two-Step RT-PCR (conventional and real-time PCR), cDNA library construction, Primer extension analysis
Citation&Papers
Ramasamy H, Kim MC,
et al., Immune response and expression analysis of cathepsin K in goldfish during Aeromonas hydrophila infection, Fish & Shellfish Immunology, 28 : 511-516, 2010
Chen XY, Lin Liu,
et al., The Arabidopsis Callose Synthase Gene GSL8 Is Required for Cytokinesis and Cell Patterning, Plant Physiology, 150 :105-113, 2009
Format
Ordering information
| Format | Cat. No. | Product | Size | Price (won) |
|---|---|---|---|---|
| Standard | DR22-R10k | DiaStar™ RT Kit, with RNase Inhibitor (RTase (M-MLV, RNase H-), Reaction Buffer (Included 10 mM dNTP), 8 mM DTT, RNase Inhibitor) |
10,000 U (200 U/㎕) (50 reaction) |
- |
| DR23-R10k | DiaStar™ RT Kit (RTase (M-MLV, RNase H-), Reaction Buffer (Included 10 mM dNTP), 8 mM DTT) |
10,000 U (200 U/㎕) |
- | |
| Pre-Mix | DR41-P096 | DiaStar™ 2X RT Pre-Mix, w/o dye, total volume 20 ㎕ reaction | 96 tube (8 strip x 12 ea) |
- |
| Single item | DR28-B50h | DiaStar™ 5X RT Reaction Buffer (Included 10 mM dNTP) | 1 ㎖ x 5 ea (1,250 reaction) |
- |
| SOR51-E20h | Solg™ RNase Inhibitor | 2,000 U (40 U/㎕) |
- | |
| SOR51-E10k | Solg™ RNase Inhibitor | 10,000 U (40 U/㎕) |
- |