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[SMP] Solg™ 2X Multiplex PCR Series

[SMP] Solg™ 2X Multiplex PCR Series

For fast Multiplex PCR set-up
Multiplex PCR up to 23-plex
Chemical-mediated Hot start enzyme
Ultra-high specificity
Amplicon size : ~ 1 kb

Solg™ 2X Multiplex PCR is designed to amplify multiple target from single template in single PCR tube using Solg™ h-Taq DNA polymerase. Chemically modified HotStart enzyme, Solg™ h-Taq DNA polymerase is useful for multiplex PCR, genotyping, microsatellite due to its high specificity. Recommended to use of amplification for less than 1 kb PCR product.

Specification

Source Thermus aquaticus
(Solg ™ h-Taq )
Amplicon size ~ 1kb
Hot start activity Yes(Chemical-mediated)
5’→ 3’ exonuclease activity yes 3’A or Blunt ends 3’A
3’→ 5’ exonuclease activit y No Error rate 12-13 bp error / 10⁶ bp

Application

Multiplex PCR, Molecular diagnosis, Allele specific PCR, Highly specific amplification of genomic and cDNA tar gets, SNP detection, SSR/MS, TA cloning

Citation&Papers

RYUK JA, CHOI GY,

et al., Application of Genetic Marker and Real-Time Polymerase Chain Reaction for Discrimination between Forsythia viridissima and Forsythia suspensa, Biological & pharmaceutical bulletin, 33 : 1133-7, 2010

Go HA, Lee MJ,

et al., Fast Identification of Korean Cattle Based on Simultaneous Dtection of Various Single Nucleotide Polymorphisms Markers by Capillary Electrophoresis, BULLETIN OF THE KOREAN CHEMICAL SOCIETY , 30 : 2141-2144, 2010

Oh DR, Cheong IC,

et al, Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds, BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 31 : 1902-1906, 2010

Format

Ordering information

Format Cat. No. Product Size Price(won)
Smart mix SMP01-M25h Solg ™ 2X Multiplex PCR Smar t mix, with dye 0.5 ㎖ x 5 ea
(100 reaction)
-
SMP02-M25h Solg ™ 2X Multiplex PCR Smar t mix, w/o dye 0.5 ㎖ x 5 ea -
Pre-Mix SMP01-P096 Solg ™ 2X Multiplex PCR Pre-Mix, with dye, total volume 30 ㎕ reaction 96 tube
(8 strip x 12 ea)
-
SMP02-P096 Solg ™ 2X Multiplex PCR Pre-Mix, w/o dye, total volume 30 ㎕ reaction 96 tube -

Manual