[SPX] Solg™ Pfu-X DNA Polymerase
For blunt-end PCR fragment amplification with fast speed & high accuracy
Ultra-high fidelity Long-range PCR
Fast elongation rate
Amplicon size : ~ 20 kb
Solg™ Pfu-X DNA polymerase is an upgraded version of Pfu DNA polymerase for long-range and high fidelity PCR. Processivity and Fidelity of Pfu-X DNA polymerase is 4 times faster and 3 times higher than Pfu polymerase. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.
Specification
| Source | Pyrococcus furiosus (Recombinant) |
Amplicon size | ~ 20 kb |
| Hot start activity | No | ||
| 5’→ 3’ exonuclease activity | No | 3’A or Blunt ends | Blunt |
| 3’→ 5’ exonuclease activit y | Yes | Error rate | 1-2 bp error / 10⁶ bp |
Application
Long-range PCR, High fidelity PCR, Site-directed mutagenesis, Blunt-end cloning
Format
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Standard | SPX16-R250 | Solg ™ Pfu-X DNA Polymerase | 250 U (200 reaction) |
- |
| SPX16-R500 | Solg ™ Pfu-X DNA Polymerase | 500 U | - | |
| SPX16-R25h | Solg ™ Pfu-X DNA Polymerase | 2,500 U (500 U x 5 ea) |
- | |
| SPX16-R50h | Solg ™ Pfu-X DNA Polymerase | 5,000 U (500 U x 10 ea) |
- | |
| Single item | SPX22-B50h | Solg ™ 10X Pfu-X Reaction Buffer | 1 ㎖ x 5 ea (1,000 reaction) |
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