[DE] DiaStar™ EF-Taq DNA Polymerase
Hot start PCR enzyme for high sensitivity & high efficiency & long fragment amplification
Long-range PCR
Antibody-mediated Hot start enzyme
High sensitivity & productivity / Low error rate
Amplicon size : ~ 20 kb
DiaStar™ EF-Taq DNA polymerase is antibody mediated HotStart DNA polymerase which is good for long-range PCR by mixing Taq DNA polymerase and Pfu DNA polymerase. It is recommended to use it when amplifying 40Kb or less.
Specification
| Source | Pyrococcus furiosus & Thermus aquaticus (Recombinant) |
Amplicon size | ~ 20 kb |
| Hot start activity | Yes (Antibody-mediated) | ||
| 5’→ 3’ exonuclease activity | Yes | 3’A or Blunt ends | 3’A |
| 3’→ 5’ exonuclease activit y | Yes (weak) | Error rate | 5-6 bp error / 10⁶ bp |
Application
Long-range PCR, Amplification of low-c opy DNA targets, High throughput hot start PCR, Highly specific amplification of genomic and cDNA tar gets, Inverse PCR, TA cloning
Format
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Standard | DE16-R250 | DiaStar™ EF-Taq DNA Polymerase | 250 U (200 reaction) |
- |
| DE16-R500 | DiaStar™ EF-Taq DNA Polymerase | 500 U | - | |
| DE16-R25h | DiaStar™ EF-Taq DNA Polymerase | 2,500 U (500 U x 5 ea) |
- | |
| DE16-R50h | DiaStar™ EF-Taq DNA Polymerase | 5,000 U (500 U x 10 ea) |
- | |
| Single item | DE22-B50h | DiaStar™ 10X EF-Taq Reaction Buffer | 1 ㎖ x 5 ea (1,000 reaction) |
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