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[SEF] Solg™ EF-Taq Polymerase

[SEF] Solg™ EF-Taq Polymerase

PCR enzyme for long fragment amplification
Long-range PCR
High productivity / Low error rate
Amplicon size : ~ 20 kb

Solg™ EF-Taq polymerase is chemical-mediated HotStart PCR enzyme, which enables multiplex and allele specific PCR for diagnostics and genotyping through initial heat activation during PCR cycling. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.

Specification

Source Thermus aquaticus
(Recombinant)		 Amplicon size ~ 20 kb
Hot start activity No
5’→ 3’ exonuclease activity Yes 3’A or Blunt ends 3’A
3’→ ’ exonuclease activit y No Error rate 5-6 bp error / 10⁶ bp

Application

Long-range PCR, High productivity PCR, Inverse PCR, TA cloning

Citation&Papers

Li CS, Zhang QG,

et al., Interleukin-27 polymorphisms are associated with inflammatory bowel diseases in a Korean population, Journal of Gastroenterology and Hepatology , 24 : 1692-1696, 2009

Fu XT, Lin H,

et al., Purification and characterization of a novel β-agarase, AgaA34, from Agarivorans albus YKW-34, Applied Microbiology and Biotechnology , 78 : 265-73, 2008

Format

Ordering information

Format Cat. No. Product Size Price(won)
Standard SEF16-R250 Solg ™ EF-Taq DNA Polymerase 250 U
(200 reaction)		 -
SEF16-R500 Solg ™ EF-Taq DNA Polymerase 500 U -
SEF16-R25h Solg ™ EF-Taq DNA Polymerase 2,500 U
(500 U x 5 ea)		 -
SEF16-R50h Solg ™ EF-Taq DNA Polymerase 5,000 U
(500 U x 10 ea)		 -
Smart mix SEF01-M50h Solg ™ 2X EF-Taq PCR Smar t mix 1, with dy e 1㎖ x 5 ea
(200 reaction)		 -
SEF02-M50h Solg ™ 2X EF-Taq PCR Smar t mix 2 (0.5X B and Doctor), with dye 1㎖ x 5 ea -
Pre-Mix SEF02-P096 Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye,
total volume 30㎕ reaction		 96 tube
(8 strip x 12 ea)		 -
SEF02-P288 Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye,
total volume 30㎕ reaction		 288 tube
(8 strip x 36 ea)		 -
SEF02-P576 Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye,
total volume 30㎕ reaction		 576 tube
(8 strip x 72 ea)		 -
Single item SEF22-B50h DiaStar™ 10X Taq Reaction Buf fer 1 ㎖ x 5 ea
(1,000 reaction)		 -

Manual

SEF01-M50h,_SEF06-M50h_Solg_2X_EF-Taq_PCR_Smart_mix_1_개정일_20181001

SEF02-M50h,_SEF07-M50h_Solg_2X_EF-Taq_PCR_Smart_mix_2_개정일_20181001

SEF02-P096,_P288,_P576_Solg_2X_EF-Taq_PCR_Pre-Mix_20190704

SEF16-R250,_R500,_R25h,_R50h_Solg_EF-Taq_DNA_Polymerase_개정일_20190523