16S/18S/26S rRNA & ITS Region Full Sequencing
- Guaranteed accuracy: 99%
- Bioinformatics analysis service support: align, blast, phylogenetic tree
- Based on SAS (SolGent Analysis Services) system, Real-time monitoring “from ordering to result check” is possible.
We Analyze the nucleotide sequence of ribosomal RNA of the Prokaryotic microorganism (Bacteria…)and Eukaryotic
microorganism (Yeast, Fungi…)and classifies and identifies microorganism species by comparing homology with GeneBank Database.
- Analysis Kit: BigDye® Terminator v3.1 cycle sequencing Kits
- Analyzer: ABI 3730XL DNA Analyzer (50 cm capillary)
Ribosomal RNA for microbiome identification
Prokaryotic Microorganism | Eukaryotic Microorganism | |
Target Gene | 16S rRNA  | ITS (18S rRNA)  |
Size | 1.5 kb | 600 ~ 800 bp |
Solgent’s Universal Primer | 27F, 337F, 518F, 785F, 518R, 783R, 805R, 1492R | ITS1, ITS4 |
Sample Preparation
Sample Type | Preparation Method | Note |
Saprophyte | Pure culture colony | .Sample Delivery: at low temperature(4℃). .Fungi & Actinomycete: Please prepare gDNA to avoid contamination by spore. |
Pathogenic Bacterium, Fungi, Actinomycete | Genomic DNA |
Analysis Progress
Retention period of sample: 2 weeks
Retention period of sequencing result : 6 months
Bioinformatics Analysis Service
We are offering Contig align, BlastN search, Phylogenetic tree service at an extra cost. Phylogenetic tree is a graph that compare multiple nucleotide sequences by using clustal W program. It could show how far evolutionary distance is from each similar sequence. We provide 4 types of phylogenetic tree that cross distance method (UPGMA & Neighbor Joining) and type of tree (Phylogram & Cladogram).
Type of Result Data
Contig align: Contigs file such as notepad, word, etc.
BlastN search: HTML file
Phylogenetic tree: HTML file